About 1,887,928 results (3,953 milliseconds)
StarrLab - Protein Extraction and Western Blotting
https://sites.google.com/a/umn.edu/starrlab/download-lab-specific-standard-operating-procedures/protein-extraction-and-western-blotting Protein Extraction and Western Blotting. 170328_SOP_Protein_Extraction_Westerns_TKS.docx · MyU | Campuses: Crookston • Duluth • Morris • Rochester • Twin ...
US9182371B2 - Microscale western blot - Google Patents
https://patents.google.com/patent/US9182371B2/en The blotting component comprises a surface proximate to one of the porous membranes forming the microfluidic channel, where the surface can include a blotting ...
Electrostatic Protein Immobilization Using Charged Polyacrylamide ...
http://feedproxy.google.com/~r/acs/ancham/~3/TgPdPMkimQk/ac3000013 Jan 26, 2012 ... We report a novel protein immobilization matrix for fully integrated microfluidic Western blotting (WB). The electrostatic immobilization ...
8. Western Blotting Protocol
https://sites.google.com/a/aymanoweida.com/www/protocols/westernblottingprotocol May 26, 2018 ... 1. Spin down lysate solution in cold room at 12500rpm for 20 minutes (protein in supernatant) 2. Remove supernatant and save into new Eppendorf tubes.
EP2302386B1 - Western blot immunoassay using hydrophilic, high ...
https://patents.google.com/patent/EP2302386B1/en Western blotting is one modification of this technique that involves the immobilization of proteins on membranes before detection using monoclonal or polyclonal ...
EP2484431A2 - Nanofiber membrane for western blot and ...
https://patents.google.com/patent/EP2484431A2/en The present invention relates to a membrane for Western blotting which has a three-dimensional open pore structure, an average pore diameter of 0.1-1.0 µm ...
StarrLab - Western Blot Dot Blots
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/western-blot-dot-blots Jan 12, 2012 ... Protocol · Dilute protein samples in dilution buffer to final protein concentration of 1 to 100 ng/µl. · Place 1 µl on a dry membrane (Qiagen ...
StarrLab - Western Blot Presentations
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/western-blot-presentations When presenting a western blot in a Starr lab meeting or presentation, include the following information: Title: Date, protein(s) and cell lysates including ...
StarrLab - Stripping and Reprobing a Western Blot Membrane
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/stripping-and-reprobing-a-western-blot-membrane Jan 12, 2012 ... Follow this protocol to strip off antibodies from a membrane in order to re-stain the membrane with a different antibody.
StarrLab - Western Blot Details
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/western-blot-details Cell names and growth conditions: Method of collecting lysates: Volume and total mass of protein loaded: Name of ladder: Type of gel: Type of membrane: ...
EP2572189B1 - Western blot analytical technique - Google Patents
https://patents.google.com/patent/EP2572189B1/en Western blotting is a labour intensive laboratory analysis method that is widely used in the life sciences to determine whether a target protein is present ...
6. Analyzing Western Blots Using ImageJ Software
https://sites.google.com/a/aymanoweida.com/www/protocols/analyzingwesternblotsusingimagejsoftware May 26, 2018 ... 1. Open image through the software. File àOpen (as TIF/JPEG) ... 2. ImageàType à8-bits ... 3. ProcessàSubtract background (use default 50.0 pixels).
StarrLab - Western blot protein detection using chemiluminescence
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/western-blot-protein-detection-using-chemiluminescence Jan 12, 2012 ... Follow this protocol to detect proteins on a PVDF or nitrocellulose filter. The first step is to "block" the membrane, which means to soak ...
WO2008103786A1 - Multi-strip western blotting procedure to ...
https://patents.google.com/patent/WO2008103786A1/en Aug 28, 2008 ... The multi-strip Western blotting procedure permits examination of up to 9 different proteins from one gel lane and transfer up to 100 different ...
Western Blotting Current Problem
https://groups.google.com/g/bionet.molbio.methds-reagnts/c/l9Go6u70Xvo May 1, 1996 ... I am having problems with Western blots and need some help. Using standard transfer buffer (Tris-glycine-methanol) in the Bio-Rad miniblot
Western Blotting Guru - Ayaz Najafov, Gerta Hoxhaj - Google Books
https://books.google.com/books/about/Western_Blotting_Guru.html?id=j8rGAQAACAAJ Jul 12, 2017 ... Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems ...
StarrLab - Chemifluorescent detection of protein on a Western Blot
https://sites.google.com/a/umn.edu/starrlab/protocols/western-blot/chemifluorescent-detection-of-protein Jan 12, 2012 ... Currently I am using chemifluorescent substrate from Amersham Pharmacia kit RPN-5783. Prepare the substrate by mixing 36 mg ECF substrate in ...
US20220010355A1 - Cell based method for determination of ...
https://patents.google.com/patent/US20220010355A1/en Cell based method for determination of botulinum toxin potency based on western blotting. Abstract. translated from. Described are cell- ...
Amy E. Herr - Google Scholar
https://scholar.google.com/citations?user=hqX6WSMAAAAJ&hl=en Single-cell western blotting. AJ Hughes, DP Spelke, Z Xu, CC Kang, DV Schaffer, AE Herr. Nature methods 11 (7), 749-755, 2014. 516, 2014. Identification of ...
WO2013051846A2 - Complex membrane for a western blot ...
https://patents.google.com/patent/WO2013051846A2/en The present invention relates to a complex membrane for a western blot including a complex structure of a PVdF nanofiber web and non-woven fabric.